Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes.

TitleStudies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes.
Publication TypeJournal Article
Year of Publication2002
AuthorsHuang JM, Huang TH, Qiu HY, Fang XW, Zhuang TG, Qiu JW
JournalAsian J Androl
Pagination209 - 12
Date PublishedSep
Accession Number12364978
KeywordsAdult, Chromosomes, Human, Hepatitis B, Hepatitis B virus, Humans, In Situ Hybridization, Fluorescence, Infectious Disease Transmission, Vertical, Male, Spermatozoa, Virus Integration

To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration.|Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.|Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random.|HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.

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