@article {699,
	title = {Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes.},
	journal = {Asian J Androl},
	volume = {4},
	year = {2002},
	month = {Sep},
	pages = {209 - 12},
	abstract = {To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration.|Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes.|Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random.|HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.},
	keywords = {Adult, Chromosomes, Human, Hepatitis B, Hepatitis B virus, Humans, In Situ Hybridization, Fluorescence, Infectious Disease Transmission, Vertical, Male, Spermatozoa, Virus Integration},
	issn = {1008-682X},
	author = {Huang,J. M. and Huang,T. H. and Qiu,H. Y. and Fang,X. W. and Zhuang,T. G. and Qiu,J. W.}
}
