Next-generation sequencing of Coccidioides immitis isolated during cluster investigation.

TitleNext-generation sequencing of Coccidioides immitis isolated during cluster investigation.
Publication TypeJournal Article
Year of Publication2011
AuthorsEngelthaler DM, Chiller T, Schupp JA, Colvin J, Beckstrom-Sternberg SM, Driebe EM, Moses T, Tembe W, Sinari S, Beckstrom-Sternberg JS, Christoforides A, Pearson JV, Carpten J, Keim P, Peterson A, Terashita D, Balajee SA
Date PublishedFeb
Accession Number21291593
KeywordsCluster Analysis, Coccidioides / cl [Classification], Coccidioides / ge [Genetics], Coccidioides / ip [Isolation & Purification], Coccidioidomycosis / di [Diagnosis], Coccidioidomycosis / ep [Epidemiology], Coccidioidomycosis / mi [Microbiology], DNA, Fungal / an [Analysis], DNA, Fungal / ge [Genetics], Genome, Fungal / ge [Genetics], Genotype, Humans, IM, Molecular Epidemiology, Mycological Typing Techniques / mt [Methods], Organ Transplantation / ae [Adverse Effects], Phylogeny, Polymorphism, Single Nucleotide, Sequence Alignment, Sequence Analysis, DNA / mt [Methods], Species Specificity

Next-generation sequencing enables use of whole-genome sequence typing (WGST) as a viable and discriminatory tool for genotyping and molecular epidemiologic analysis. We used WGST to confirm the linkage of a cluster of Coccidioides immitis isolates from 3 patients who received organ transplants from a single donor who later had positive test results for coccidioidomycosis. Isolates from the 3 patients were nearly genetically identical (a total of 3 single-nucleotide polymorphisms identified among them), thereby demonstrating direct descent of the 3 isolates from an original isolate. We used WGST to demonstrate the genotypic relatedness of C. immitis isolates that were also epidemiologically linked. Thus, WGST offers unique benefits to public health for investigation of clusters considered to be linked to a single source.

Alternate JournalEmerg.Infect.Dis.
Notify Library Reference ID1790

Related Incidents