| Title | Factors affecting banking quality of umbilical cord blood for transplantation |
| Publication Type | Journal Article |
| Year of Publication | 2010 |
| Authors | Yang H, Loutfy MR, Mayerhofer S, Shuen P |
| Journal | Transfusion |
| Date Published | Aug 16 |
| ISSN | 1537-2995 (Electronic) 0041-1132 (Linking) |
| Accession Number | 20723167 |
| Abstract | BACKGROUND: The most important objective for cord blood banks is to store cord blood units of high quality, which is determined by total nucleated cells (TNCs) and CD34+ cells. Determining the factors affecting the stored life-saving cells would be beneficial to the field. STUDY DESIGN AND METHODS: A total of 4930 cord blood units were collected between January 2007 and October 2009 and processed using a double extraction technique to sediment red blood cells with variable centrifugation time determined by the formula CT = KL - M, where CT is centrifuge time, K is 7.7227, M is 29.742, and L is ln (volume of cord blood with anticoagulant). The recovery rate of TNCs and other relevant factors affecting banking quality were analyzed. RESULTS: The mean recovery rate of TNCs was 97.7 +/- 2.5% with 0.04% (2/4930) units below 80% and 10.8% (532/4930) units below 95%. The TNCs per unit was affected by gestation duration (p |
| DOI | 10.1111/j.1537-2995.2010.02826.x |
| Notify Library Reference ID | 1661 |
