@article {1661,
	title = {Factors affecting banking quality of umbilical cord blood for transplantation},
	journal = {Transfusion},
	year = {2010},
	note = {Journal article Transfusion Transfusion. 2010 Aug 16.},
	month = {Aug 16},
	edition = {40411},
	abstract = {BACKGROUND: The most important objective for cord blood banks is to store cord blood units of high quality, which is determined by total nucleated cells (TNCs) and CD34+ cells. Determining the factors affecting the stored life-saving cells would be beneficial to the field. STUDY DESIGN AND METHODS: A total of 4930 cord blood units were collected between January 2007 and October 2009 and processed using a double extraction technique to sediment red blood cells with variable centrifugation time determined by the formula CT = KL - M, where CT is centrifuge time, K is 7.7227, M is 29.742, and L is ln (volume of cord blood with anticoagulant). The recovery rate of TNCs and other relevant factors affecting banking quality were analyzed. RESULTS: The mean recovery rate of TNCs was 97.7 +/- 2.5\% with 0.04\% (2/4930) units below 80\% and 10.8\% (532/4930) units below 95\%. The TNCs per unit was affected by gestation duration (p < 0.01), sex of infant (p < 0.01), mode of delivery (p < 0.01), collection method (p < 0.01), and ethnicity (p < 0.001). The number of postprocessing CD34+ cells was affected only by sex of the infant (p < 0.05). The viability of nucleated cells after processing was 94.8 +/- 4.8\% and was affected by the number of hours between collection and processing (p < 0.01). In contrast, the viability of CD34+ cells was 99.5 +/- 1.0\% (n = 30) when samples with low viability of TNCs were assessed. The results did not reveal a significant correlation (r = 0.07, p = 0.38). CONCLUSION: The double extraction technique provides a high and consistent recovery of TNCs, which ensures that more life-saving cells will be banked for transplants.},
	issn = {1537-2995 (Electronic) 0041-1132 (Linking)},
	doi = {TRF2826 [pii] 10.1111/j.1537-2995.2010.02826.x},
	author = {Yang,H. and Loutfy,M. R. and Mayerhofer,S. and Shuen,P.}
}
