Improved preparation and preservation of human keratoplasty lenticules

TitleImproved preparation and preservation of human keratoplasty lenticules
Publication TypeJournal Article
Year of Publication2003
AuthorsSalvalaio G, Fasolo A, Bruni A, Frigo AC, Favaro E, Ponzin D
JournalOphthalmic Res
Pagination313 - 8
Date PublishedNov-Dec
ISSN0030-3747 (Print) 0030-3747 (Linking)
Accession Number14688420
Keywords*Cornea, Cell Survival / physiology, Chondroitin Sulfates, Complex Mixtures, Corneal Stroma / *cytology, Cryopreservation / *methods, Cryoprotective Agents / therapeutic use, Culture Media, Serum-Free, Debridement, Dextrans, Fibroblasts / *cytology, Gentamicins, Humans, Keratoplasty, Penetrating, Organ Preservation / *methods, Staining and Labeling / methods

PURPOSE: To improve the preparation of lenticules from human cornea and to obtain their preservation without loss of viable keratocytes. METHODS: The epithelium was manually removed after bathing the surface of the cornea with a solution of trypsin and EDTA. Lenticules were prepared by microkeratome resection and viable keratocytes were visualized by staining with thiazolyl blue (MTT). RESULTS: The pretreatment with trypsin-EDTA allowed the removal of the epithelium without damage to the keratocytes and the stroma. When these lenticules were incubated in Optisol-GS for 7 days at 4 degrees C, they showed a limited thickness increase and a preservation of keratocyte viability. CONCLUSION: This procedure allows the preparation of lenticules with viable keratocytes that can be preserved in the cold for at least 1 week.

DOI10.1159/000074069 ORE2003035006313 [pii]
Notify Library Reference ID1351

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