@article {1351,
	title = {Improved preparation and preservation of human keratoplasty lenticules},
	journal = {Ophthalmic Res},
	volume = {35},
	year = {2003},
	note = {Salvalaio, Gianni Fasolo, Adriano Bruni, Alessandro Frigo, Anna Chiara Favaro, Elisa Ponzin, Diego Switzerland Ophthalmic research Ophthalmic Res. 2003 Nov-Dec;35(6):313-8.},
	month = {Nov-Dec},
	pages = {313 - 8},
	edition = {37978},
	abstract = {PURPOSE: To improve the preparation of lenticules from human cornea and to obtain their preservation without loss of viable keratocytes. METHODS: The epithelium was manually removed after bathing the surface of the cornea with a solution of trypsin and EDTA. Lenticules were prepared by microkeratome resection and viable keratocytes were visualized by staining with thiazolyl blue (MTT). RESULTS: The pretreatment with trypsin-EDTA allowed the removal of the epithelium without damage to the keratocytes and the stroma. When these lenticules were incubated in Optisol-GS for 7 days at 4 degrees C, they showed a limited thickness increase and a preservation of keratocyte viability. CONCLUSION: This procedure allows the preparation of lenticules with viable keratocytes that can be preserved in the cold for at least 1 week.},
	keywords = {*Cornea, Cell Survival / physiology, Chondroitin Sulfates, Complex Mixtures, Corneal Stroma / *cytology, Cryopreservation / *methods, Cryoprotective Agents / therapeutic use, Culture Media, Serum-Free, Debridement, Dextrans, Fibroblasts / *cytology, Gentamicins, Humans, Keratoplasty, Penetrating, Organ Preservation / *methods, Staining and Labeling / methods},
	issn = {0030-3747 (Print) 0030-3747 (Linking)},
	doi = {10.1159/000074069 ORE2003035006313 [pii]},
	author = {Salvalaio,G. and Fasolo,A. and Bruni,A. and Frigo,A. C. and Favaro,E. and Ponzin,D.}
}
