| Title | Improved preparation and preservation of human keratoplasty lenticules |
| Publication Type | Journal Article |
| Year of Publication | 2003 |
| Authors | Salvalaio G, Fasolo A, Bruni A, Frigo AC, Favaro E, Ponzin D |
| Journal | Ophthalmic Res |
| Volume | 35 |
| Issue | 6 |
| Pagination | 313 - 8 |
| Date Published | Nov-Dec |
| ISSN | 0030-3747 (Print) 0030-3747 (Linking) |
| Accession Number | 14688420 |
| Keywords | *Cornea, Cell Survival / physiology, Chondroitin Sulfates, Complex Mixtures, Corneal Stroma / *cytology, Cryopreservation / *methods, Cryoprotective Agents / therapeutic use, Culture Media, Serum-Free, Debridement, Dextrans, Fibroblasts / *cytology, Gentamicins, Humans, Keratoplasty, Penetrating, Organ Preservation / *methods, Staining and Labeling / methods |
| Abstract | PURPOSE: To improve the preparation of lenticules from human cornea and to obtain their preservation without loss of viable keratocytes. METHODS: The epithelium was manually removed after bathing the surface of the cornea with a solution of trypsin and EDTA. Lenticules were prepared by microkeratome resection and viable keratocytes were visualized by staining with thiazolyl blue (MTT). RESULTS: The pretreatment with trypsin-EDTA allowed the removal of the epithelium without damage to the keratocytes and the stroma. When these lenticules were incubated in Optisol-GS for 7 days at 4 degrees C, they showed a limited thickness increase and a preservation of keratocyte viability. CONCLUSION: This procedure allows the preparation of lenticules with viable keratocytes that can be preserved in the cold for at least 1 week. |
| DOI | 10.1159/000074069 ORE2003035006313 [pii] |
| Notify Library Reference ID | 1351 |
Improved preparation and preservation of human keratoplasty lenticules
Related Incidents
- 721 - Cornea lenticule of inadequate quality - Cornea