| Title | PCR-detected hepatitis C virus RNA associated with human zona-intact oocytes collected from infected women for ART. |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Papaxanthos-Roche A, Trimoulet P, Commenges-Ducos M, Hocké C, Fleury HJ, Mayer G |
| Journal | Hum Reprod |
| Volume | 19 |
| Issue | 5 |
| Pagination | 1170 - 5 |
| Date Published | May |
| ISSN | 0268-1161 |
| Accession Number | 15044403 |
| Keywords | Adult, Female, Follicular Fluid, Hepacivirus, Hepatitis C, Humans, Oocytes, Polymerase Chain Reaction, Reproductive Techniques, Assisted, Risk Factors, RNA, Viral, Viral Load, Zona Pellucida |
| Abstract | The aim of this study was to investigate the susceptibility of human oocytes from hepatitis C virus (HCV) RNA-positive women to HCV contamination during assisted reproductive technology (ART).|A reverse transcriptase-PCR assay was used to test for the presence of HCV RNA associated with 24 unfertilized oocytes 48 h after follicular fluid aspiration in 10 IVF attempts (seven conventional IVF and three ICSI). Negative and positive controls (10 unfertilized oocytes from HCV-negative women and 20 unfertilized oocytes artificially contaminated with HCV RNA-positive plasma; HCV RNA was also quantified in plasma and follicular fluid) were included.|HCV RNA was associated with 17/24 (70.8%) oocytes (6/7 after ICSI and 11/17 after conventional IVF) and was found in 19/20 (95%) follicular fluid samples. A weak correlation was found between plasma and follicular fluid HCV RNA loads (r = 0.73, P |
| DOI | 10.1093/humrep/deh220 |
| Notify Library Reference ID | 1135 |
