@article {1135,
	title = {PCR-detected hepatitis C virus RNA associated with human zona-intact oocytes collected from infected women for ART.},
	journal = {Hum Reprod},
	volume = {19},
	year = {2004},
	month = {May},
	pages = {1170 - 5},
	abstract = {The aim of this study was to investigate the susceptibility of human oocytes from hepatitis C virus (HCV) RNA-positive women to HCV contamination during assisted reproductive technology (ART).|A reverse transcriptase-PCR assay was used to test for the presence of HCV RNA associated with 24 unfertilized oocytes 48 h after follicular fluid aspiration in 10 IVF attempts (seven conventional IVF and three ICSI). Negative and positive controls (10 unfertilized oocytes from HCV-negative women and 20 unfertilized oocytes artificially contaminated with HCV RNA-positive plasma; HCV RNA was also quantified in plasma and follicular fluid) were included.|HCV RNA was associated with 17/24 (70.8\%) oocytes (6/7 after ICSI and 11/17 after conventional IVF) and was found in 19/20 (95\%) follicular fluid samples. A weak correlation was found between plasma and follicular fluid HCV RNA loads (r = 0.73, P < 0.001).|HCV associated with unfertilized oocytes surrounded by their intact zona pellucida from anti-HCV antibody-positive and viraemic women undergoing ART raises questions concerning the safe management of medically assisted procreation for these women and good practice of oocyte/embryo cryopreservation and donation.},
	keywords = {Adult, Female, Follicular Fluid, Hepacivirus, Hepatitis C, Humans, Oocytes, Polymerase Chain Reaction, Reproductive Techniques, Assisted, Risk Factors, RNA, Viral, Viral Load, Zona Pellucida},
	issn = {0268-1161},
	doi = {deh220 [pii] 10.1093/humrep/deh220},
	author = {Papaxanthos-Roche,A. and Trimoulet,P. and Commenges-Ducos,M. and Hock{\'e},C. and Fleury,H. J. and Mayer,G.}
}
