Origin of adenocarcinoma in a transplanted liver determined by microsatellite analysis

TitleOrigin of adenocarcinoma in a transplanted liver determined by microsatellite analysis
Publication TypeJournal Article
Year of Publication2002
AuthorsKakar S, Burgart LJ, Charlton MR, Saito Y, Halling K, Thibodeau SN
JournalHum Pathol
Volume33
Issue4
Pagination435 - 6
Date PublishedApr
Accession Number12055679
Keywords*Liver Transplantation, *Microsatellite Repeats, *Tissue Donors, Adenocarcinoma / *genetics / pathology, Adult, Biopsy, DNA, Neoplasm / analysis, Hepatitis C / complications / surgery, Humans, Liver / pathology, Liver Cirrhosis, Alcoholic / complications / surgery, Liver Neoplasms / *genetics / pathology, Male, Polymerase Chain Reaction
Abstract

Inadvertent transmission of neoplastic cells from an organ donor can occur at the time of transplantation. Determination of recipient versus donor origin of a tumor is crucial for patient management. This report illustrates the use of microsatellite (MS) analysis to determine the origin of adenocarcinoma arising in a liver transplant. The study patient was a 42-year-old male who had received a liver transplant for hepatitis C and alcohol-related cirrhosis. At the 1-year follow-up visit, a 1.5-cm liver mass was identified during routine ultrasound of the vascular anastamoses. A liver biopsy showed a moderately differentiated adenocarcinoma. Tumor, donor, and recipient DNA were isolated from the paraffin-embedded liver biopsy, pretransplant donor liver biopsy, and the explant liver tissue, respectively. MS analysis was performed by polymerase chain reaction using 5 markers: D5S346, ACTC, D2S123, D18S34, and TP53. The allelic patterns of tumor DNA were identical to those of donor DNA and were distinct from the DNA profile of the recipient. The use of MS analysis clearly established that the adenocarcinoma was of donor origin.

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