Quantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility.

TitleQuantitative and qualitative analysis of microorganisms in an assisted reproductive technology facility.
Publication TypeJournal Article
Year of Publication2008
AuthorsHerlong JL, Reubish K, Higdon HL, Boone WR
JournalFertil Steril
Pagination847 - 53
Date PublishedApr
Accession Number17678906
KeywordsAir Microbiology, Andrology, Bacteria, Colony Count, Microbial, Embryo Culture Techniques, Environment, Controlled, Environmental Monitoring, Equipment Contamination, Fungi, Humans, Incubators, Laboratories, Hospital, Prospective Studies, Reproductive Techniques, Assisted, Ventilation

OBJECTIVE: To perform a quantitative and qualitative analysis of the environmental microorganisms associated with two assisted reproductive technology (ART) laboratories. DESIGN: Prospective descriptive study. SETTING: Two ART laboratories at Greenville Hospital System (GHS) University Medical Center in Greenville, South Carolina. PATIENT(S): None. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Quantitative and qualitative analysis of colony-forming units (CFU; bacteria and fungi) and particles from an embryology clean room and an andrology laboratory. RESULT(S): Within these laboratories, three of the sites sampled differed significantly in the mean number of CFU per plate. These sites included external laboratory surfaces, laboratory room air, and air within cell-culture incubators. The mean number of viable CFU per cubic meter of laboratory room air differed significantly between the two laboratories. A total of 23 unique microbial species were isolated from the andrology laboratory, whereas 10 unique species were isolated from the embryology clean room. CONCLUSION(S): The data herein suggest that the increased positive control measures used in the embryology clean room are effective in minimizing environmental biologic contamination.

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