Donor origin of BKV replication after kidney transplantation.

TitleDonor origin of BKV replication after kidney transplantation.
Publication TypeJournal Article
Year of Publication2014
AuthorsSchmitt C, Raggub L, Linnenweber-Held S, Adams O, Schwarz A, Heim A
JournalJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology//J Clin Virol
Pagination120 - 5
Date Published2014
ISBN Number1873-5967
Other Numberscx0, 9815671
Keywords*BK Virus/ip [Isolation & Purification], *Kidney Transplantation/ae [Adverse Effects], *Polyomavirus Infections/tm [Transmission], *Tumor Virus Infections/tm [Transmission], Adolescent, Adult, Aged, Antigens, Viral, Tumor/ge [Genetics], BK Virus/ge [Genetics], Capsid Proteins/ge [Genetics], DNA, Viral/ch [Chemistry], DNA, Viral/ge [Genetics], Female, Genotype, Humans, Male, Middle Aged, Molecular Sequence Data, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Tissue Donors, Urine/vi [Virology], Young Adult

BACKGROUND: BK virus associated nephropathy (BKVN) leads to renal allograft dysfunction and loss. However, it is still unclear whether BKV replication in the transplant recipient is a result of reactivation in the recipient's native kidneys or whether BKV originates from the donor kidney., STUDY DESIGN: Urine of 249 donor/recipient pairs was investigated for the presence of BKV-DNA by qPCR before living transplantation (Tx) and consecutively after Tx. In BKV positive samples, the VP1 typing region (TR) and, in case of the presence of sufficient amount of DNA, the complete VP1 gene, the NCCR and a fragment of the Large T-antigen were sequenced and compared between donors and corresponding recipients before and after Tx., RESULTS: In 20 pairs, sequencing of the BKV TR succeeded in donors and corresponding recipients after Tx. The derived sequences were completely identical in donor and post-Tx recipient samples. For comparison, identical TR sequences were detected in only 24% of 1068 randomly assembled pairs. This difference was statistically highly significant (p

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