Herpes simplex virus in primary graft failure

TitleHerpes simplex virus in primary graft failure
Publication TypeJournal Article
Year of Publication1997
AuthorsCockerham GC, Krafft AE, McLean IW
JournalArch Ophthalmol
Pagination586 - 9
Date PublishedMay
ISSN0003-9950 (Print) 0003-9950 (Linking)
Accession Number9152124
Keywords*Corneal Transplantation, Adult, Blotting, Southern, Cornea / pathology / *virology, DNA Primers / chemistry, DNA, Viral / analysis, Female, Graft Rejection / pathology / *virology, Herpesvirus 1, Human / genetics / *isolation & purification, Humans, Keratitis, Herpetic / *etiology / pathology, Middle Aged, Polymerase Chain Reaction

OBJECTIVE: To determine the frequency of herpes simplex virus type 1 (HSV-1) in primary corneal graft failures. METHODS: The clinical data submitted about all cases of corneal graft failure on file at the Armed Forces Institute of Pathology, Washington, DC, from the last 25 years (1970-1995) were evaluated. Cases that met the definition of primary graft failure were examined microscopically and analyzed using polymerase chain reaction (PCR) for the DNA of HSV-1. RESULTS: Three (2.8%) of the 106 cases of graft failure were primary graft failures. The DNA from 2 of the 3 corneal buttons was amplifiable by PCR analysis and results of the PCR analysis and Southern blotting were positive for HSV-1. None of the results of the PCR analysis and Southern blotting of the corneal buttons from the 3 graft failures occurring later than 30 days were positive for HSV-1. Results of the PCR analysis and Southern blotting indicated that 2 of 3 corneal buttons in the control group of clinically suspected herpetic keratitis were positive for HSV-1. The cornea from the first case of primary graft failure showed acute inflammation with stromal necrosis. The cornea in the second case had loss of endothelium without inflammation. CONCLUSIONS: The finding of DNA from HSV-1 in corneal buttons from 2 cases of primary graft failure supports similar observations by Cleator et al and suggests that HSV-1 may be pathogenic in some cases of primary graft failure. A larger study is needed to determine if HSV-1 is a causative factor in primary graft failure.

URLinternal-pdf://cockerham - HSV-0394329089/cockerham - HSV.pdf
Notify Library Reference ID358

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