Microbiologic study of organ-cultured donor corneas

TitleMicrobiologic study of organ-cultured donor corneas
Publication TypeJournal Article
Year of Publication1998
AuthorsBorderie VM, Laroche L
Pagination120 - 3
Date PublishedJul 15
ISSN0041-1337 (Print) 0041-1337 (Linking)
Accession Number9679833
Keywords*Tissue Donors, Adult, Aged, Aged, 80 and over, Bacteria / isolation & purification, Bacterial Physiological Phenomena, Cornea / *microbiology, Cryopreservation, Drug Resistance, Microbial / physiology, Fungi / isolation & purification / physiology, Humans, Middle Aged, Organ Culture Techniques, Risk Factors, Time Factors

BACKGROUND: Our purpose was to evaluate the sterility of organ-cultured human donor corneas at the time of surgery. METHODS: We studied 603 organ-cultured corneas. Of these 603 corneas, 409 (68%) were grafted and 69 (11%) were contaminated during storage. RESULTS: Contamination during preservation was either bacterial (65%) or fungal (35%). None of the tested antibiotics were effective against all of the 45 isolated bacteria. The risk of contamination decreased with death-to-organ culture time (P=0.008) and was higher for corneas excised in situ than for those enucleated (P=0.02). Corneoscleral rims were sterile in 99.3% of the grafted corneas. Deswelling media were sterile in 100% of cases. A 19- to 53-fold decrease in the percentage of rim contamination was assessed with organ culture as compared with hypothermic storage (previous studies, P

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