Successful donor cell engraftment in a recipient of bone marrow from a cadaveric donor

TitleSuccessful donor cell engraftment in a recipient of bone marrow from a cadaveric donor
Publication TypeJournal Article
Year of Publication1986
AuthorsBlazar BR, Lasky LC, Perentesis JP, Watson KV, Steinberg SE, Filipovich AH, Orr HT, Ramsay NK
Pagination1655 - 60
Date PublishedJun
Type of ArticleCase Reports Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
ISSN0006-4971 (Print) 0006-4971 (Linking)
Accession Number3518833
Keywords*Bone Marrow Transplantation, Cadaver, Child, Erythrocytes / analysis, Genotype, Graft vs Host Disease, Humans, Leukemia, Lymphoid / *therapy, Male, Phenotype, Polymorphism, Genetic, Stem Cells / cytology

A 12-year-old male with acute lymphocytic leukemia received donor bone marrow from his histocompatible father whose marrow was harvested 40 minutes postmortem after he suffered a myocardial infarction. The marrow was stored in liquid nitrogen for 17 days prior to infusion into the recipient. Trypan blue viability was greater than 99% for the fresh marrow. Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E, 10% of the CFU-E, and 17% of the CFU-GM were spared during the cryopreservation period. Posttransplantation, the recipient had a leukocyte count greater than 10(3)/microL by day 26. Southern blotting analysis documented the donor origin of the peripheral blood mononuclear cells and granulocytes isolated 46 days posttransplantation. Unfortunately, the patient died of complications relating to graft-v-host disease 67 days following transplantation. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells and is the first reported case of documented leukocyte engraftment in a recipient of cadaveric marrow.

Alternate JournalBlood
Notify Library Reference ID186

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