Hepatitis B transmission from contaminated cryopreservation tank

TitleHepatitis B transmission from contaminated cryopreservation tank
Publication TypeJournal Article
Year of Publication1995
AuthorsTedder RS, Zuckerman MA, Goldstone AH, Hawkins AE, Fielding A, Briggs EM, Irwin D, Blair S, Gorman AM, Patterson KG
Pagination137 - 40
Date PublishedJul 15
ISSN0140-6736 (Print) 0140-6736 (Linking)
Accession Number7603227
Keywords*Cryopreservation / standards, *Equipment Contamination, Acute Disease, Adult, Bone Marrow Cells, DNA, Viral / genetics, Drug Contamination, Female, Hematopoietic Stem Cells, Hepatitis B / *transmission / virology, Hepatitis B Surface Antigens / analysis, Hepatitis B virus / genetics / isolation & purification, Humans, Male, Middle Aged, Nitrogen / analysis, Tissue Donors

Over a 25-month period, six multiply transfused patients undergoing cytotoxic treatment for haematological or other malignant disorders developed icteric acute hepatitis B virus (HBV) infection. Bone marrow or peripheral-blood stem cells had been harvested from all six patients and stored in the same cryopreservation tank for possible future transplantation. Human DNA, HBsAg, and HBV DNA with sequences identical to those from four patients with related infections were subsequently found in the liquid nitrogen. Leakage of the cryopreservation bags used to store bone marrow harvested from the first patient when acutely infected with HBV led to contamination of the tank and its contents with HBV and subsequent transmission to patients after transplantation. This incident emphasises the continuing need to screen donors of tissue to be cryopreserved for bloodborne virus infections. It also reinforces the requirement for primary containers used to cryopreserve human tissue to be sealed in a way which prevents exchange of material between the specimen and the liquid nitrogen.

Notify Library Reference ID1530

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