Donor origin of neuroendocrine carcinoma in 2 transplant patients determined by molecular cytogenetics

TitleDonor origin of neuroendocrine carcinoma in 2 transplant patients determined by molecular cytogenetics
Publication TypeJournal Article
Year of Publication2000
AuthorsBaehner R, Magrane G, Balassanian R, Chang C, Millward C, Wakil AE, Osorio RW, Waldman FM
JournalHum Pathol
Pagination1425 - 9
Date PublishedNov
Accession Number11112220
Keywords*Tissue Donors, Adult, Carcinoma, Neuroendocrine / *etiology / genetics / pathology, Carcinoma, Small Cell / *etiology / genetics / pathology, DNA, Neoplasm / analysis, Humans, In Situ Hybridization, Fluorescence, Kidney Neoplasms / *etiology / genetics / pathology, Kidney Transplantation / adverse effects, Liver Neoplasms / *etiology / genetics / pathology, Liver Transplantation / adverse effects, Male, Microsatellite Repeats, Organ Transplantation / *adverse effects, Polymerase Chain Reaction, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., X Chromosome

Organ transplant recipients have an increased tumor incidence owing to their immunocompromised state. The origin of such tumors, whether donor or recipient, will have a clinical impact on decision-making concerning immunosuppressive therapy, retransplantation, and for recipients of other organs from the same donors. We report molecular cytogenetic determination of donor origin in 2 cases of small-cell neuroendocrine carcinoma developing in sex-mismatched transplant recipients (kidney and liver). Fluorescence in situ hybridization (FISH) analysis was performed on liver core needle biopsy material from the liver transplant patient and on liver fine needle aspiration cytopreparations from the kidney transplant patient. The results for the liver transplant patient were confirmed with microsatellite allelic analysis and with comparative genomic hybridization. In both cases, FISH showed the presence of only X chromosomes within the tumor cells, indicating the donor origin of the neoplasms. FISH is an excellent method to determine neoplastic origin in sex-mismatched transplant patients. HUM PATHOL 31:1425-1429.

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