| Title | Persistence of human parvovirus B19 in multipotent mesenchymal stromal cells expressing the erythrocyte P antigen: implications for transplantation |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | Sundin M, Lindblom A, Orvell C, Barrett AJ, Sundberg B, Watz E, Wikman A, Broliden K, Le Blanc K |
| Journal | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation |
| Volume | 14 |
| Issue | 10 |
| Pagination | 1172 - 9 |
| Date Published | Oct |
| Type of Article | Research Support, Non-U.S. Gov't |
| ISSN | 1523-6536 (Electronic) 1083-8791 (Linking) |
| Accession Number | 18804048 |
| Keywords | Coculture Techniques, DNA, Viral / analysis, Humans, Mass Screening, Mesenchymal Stem Cell Transplantation / adverse effects, Mesenchymal Stem Cells / *immunology / *virology, P Blood-Group System / *immunology, Parvoviridae Infections / *transmission, Parvovirus B19, Human / *isolation & purification, Retrospective Studies, Tissue Donors |
| Abstract | Multipotent mesenchymal stromal cells (MSCs) are used to improve the outcome of hematopoietic stem cell transplantation (HCST) and in regenerative medicine. MSCs may harbor persistent viruses that may compromise their clinical benefit, however. Retrospectively screened, 1 of 20 MSCs from healthy donors contained parvovirus B19 (B19) DNA. MSCs express the B19 receptor (P antigen/globoside) and a co-receptor (Ku 80) and can transmit B19 to bone marrow cells in vitro, suggesting that the virus can persist in the marrow stroma of healthy individuals. Two patients undergoing HSCT received the B19-positive MSCs as treatment for graft-versus-host disease; neither developed viremia nor symptomatic B19 infection. These findings demonstrate for the first time that persistent B19 in MSCs can infect hematopoietic stem cells and underscore the importance of monitoring B19 transmission by MSC products. |
| DOI | 10.1016/j.bbmt.2008.08.003 |
| Alternate Journal | Biol Blood Marrow Transplant |
| Notify Library Reference ID | 1499 |
