Microbial contamination

Record number:

1123

Adverse Occurrence type:

MPHO Type:

Estimated frequency:

95 cases/13.977 cycles (0,68%) (1/3 from semen used for fertilization) all in IVF. None in ICSI procedures. Others' data: 0,69% -0,35% 3,3% (5/156 IVF) but 4/5 underwent transfer with subsequent pregnancy.

Alerting signals, symptoms, evidence of occurrence:

Loss or damage to embryos due to contamination of embryo culture.

Demonstration of imputability or root cause:

Contamination of culture media by E. coli. Analysis requested for straws and vaginal sampling.The fact that in ICSI procedures the incidence of infection is null demonstrates clearly that the origin is not intra laboratory, so lab air is not relevant.

Keywords:

embryo

semen

contamination

E. Coli (Escherichia coli)

straws

infection

laboratory

ICSI (intracytoplasmic sperm injection)

partner

MAR (medically assisted reproduction)

conventional in vitro insemination