%0 Journal Article %J Hum Reprod %D 2007 %T Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples %A Savasi,V. %A Ferrazzi,E. %A Lanzani,C. %A Oneta,M. %A Parrilla,B. %A Persico,T. %K *HIV Seropositivity %K *Reproductive Techniques, Assisted %K Acquired Immunodeficiency Syndrome / *prevention & control %K Female %K Fertilization in Vitro %K Humans %K Insemination, Artificial, Homologous %K Male %K Pregnancy %K Pregnancy Rate %K Specimen Handling / *methods %K Sperm Injections, Intracytoplasmic %K Spermatozoa / *virology %K Tissue Donors %K Treatment Outcome %K Viral Load %X BACKGROUND: To evaluate the safety of sperm washing and assisted reproduction technique (ART) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (HIV-1)-positive male. METHODS: Sperm washing was performed and checked by RT-PCR on each semen sample before its fresh usage. Intrauterine insemination (IUI) or IFV/ICSI was offered according to fertility profile of each couple. Non-infected women underwent HIV testing 2 weeks before each procedure and for up to 6 months after. RESULTS: Seven hundred and forty-one couples entered the study of a possible 2011 serodiscordant couples counselled over 4 years. Superovulation and IUI were performed in 581 couples, where the pregnancy rate per cycle and pregnancy rate per couple were 19 and 78%, respectively, with multiple pregnancy rate being 4%. One hundred and sixty couples were treated by IVF/ICSI, where pregnancy rate per cycle and per couple were 22 and 41%, respectively, with multiple pregnancy rate being 10%. All female partners were still HIV-1 negative at follow-up. CONCLUSION: Sperm washing within a programme of reproductive counselling was proved to be safe in this large series of serodiscordant couples. The overall pregnancy rate (70.3%), independent of the procedure used (IUI or IVF/ICSI), justifies the effort of the medical team in setting up and implementing dedicated centres and of the individual patient in seeking a safe pregnancy. %B Hum Reprod %7 39038 %V 22 %P 772 - 7 %8 Mar %G eng %N 3 %M 17107974 %R del422 [pii] 10.1093/humrep/del422