TY - JOUR T1 - A new blood group antigen is defined by anti-CD59, detected in a CD59-deficient patient. JF - Transfusion//Transfusion Y1 - 2014 A1 - Anliker, Markus A1 - von Zabern, Inge A1 - Hochsmann, Britta A1 - Kyrieleis, Henriette A1 - Dohna-Schwake, Christian A1 - Flegel, Willy A A1 - Schrezenmeier, Hubert A1 - Weinstock, Christof KW - *Anemia, Hemolytic/im [Immunology] KW - *Antigens, CD59/im [Immunology] KW - *Blood Group Antigens/im [Immunology] KW - *Hemoglobinuria/im [Immunology] KW - *Isoantibodies/bl [Blood] KW - Anemia, Hemolytic/ge [Genetics] KW - Antigens, CD59/ge [Genetics] KW - Blood Group Antigens/ge [Genetics] KW - Child, Preschool KW - Female KW - Frameshift Mutation KW - Hemoglobinuria/ge [Genetics] KW - Humans AB - BACKGROUND: CD59 is a cell surface glycoprotein of approximately 20kDa limiting the lytic activity of the terminal complement complex C5b-9. Although CD59 is known as a red blood cell (RBC) antigen defined by monoclonal antibodies, it so far has not been identified as a blood group antigen, since the description of a human alloantibody was missing. In this study we show the presence of an anti-CD59 in a patient affected by a homozygous CD59 deficiency., STUDY DESIGN AND METHODS: RBC CD59 and CD55 were determined by flow cytometry or by the column agglutination technique using monoclonal antisera. Commercially available His-tagged recombinant soluble CD59 protein was used to inhibit anti-CD59., RESULTS: Seven cases of an isolated CD59 deficiency due to three distinct null alleles of the CD59 gene have been published so far. Recently we described the CD59-null allele c.146delA in a young child of heterozygous parents. Her plasma contained an alloantibody directed against the high-prevalence RBC antigen CD59. The antibody specificity was identified using soluble recombinant human CD59 protein, which blocked the reactivity of the patient's antibody and of monoclonal anti-CD59 but not of monoclonal anti-CD55. In addition, RBC alloantibodies such as anti-K, anti-C, anti-c, or anti-Fy(a) remained unaffected. Therefore, inhibition by recombinant CD59 is a useful diagnostic tool to detect alloantibodies in the presence of anti-CD59., CONCLUSION: This is the first demonstration of a human anti-CD59 alloantibody, which defines CD59 as an RBC blood group antigen. CD59 represents a candidate for a new blood group system.Copyright © 2014 AABB. M1 - wdn, 0417360 PB - Anliker,Markus. Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service Baden-Wurttemberg-Hessen, Ulm, Germany; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany. CY - United States VL - 54 SN - 1537-2995 CP - 7 L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=medl&NEWS=N&AN=24383981 ID - 4411 ER -