TY - JOUR T1 - Transmission of cytomegalovirus (CMV) infection by leukoreduced blood products not tested for CMV antibodies: a single-center prospective study in high-risk patients undergoing allogeneic hematopoietic stem cell transplantation (CME). JF - //Transfusion Y1 - 2011 A1 - Thiele, Thomas A1 - Kruger, William A1 - Zimmermann, Kathrin A1 - Ittermann, Till A1 - Wessel, Antje A1 - Steinmetz, Ivo A1 - Dolken, Gottfried A1 - Greinacher, Andreas AB - BACKGROUND: Measures to prevent transfusion-transmitted cytomegalovirus (TT-CMV) infection after hematopoietic stem cell transplantation (HSCT) include transfusion of CMV antibody-negative blood units and/or transfusion of leukoreduced cellular blood products. We assessed the incidence of TT-CMV in CMV-seronegative patients receiving CMV-seronegative HSC transplants, who were transfused with leukoreduced cellular blood products not tested for anti-CMV., STUDY DESIGN AND METHODS: In a prospective observational study between 1999 and 2009, all HSCT patients received leukoreduced cellular blood products not tested for anti-CMV. Patients were screened for CMV serostatus and CMV-negative recipients of CMV-negative transplants were systematically monitored for TT-CMV clinically and by CMV nucleic acid testing. Anti-CMV antibodies (immunoglobulin [Ig]G and IgM) were assessed after three time intervals (Interval 1, study inclusion to Day +30 after HSCT; Interval 2, Day +30-Day +100; Interval 3, after Day +100)., RESULTS: Among 142 patients treated with allogeneic HSCT, 23 CMV-negative donor-patient pairs were identified. These 23 patients received 1847 blood products from 3180 donors. All patients remained negative for CMV DNA and none developed CMV-associated clinical complications. This results in a risk for TT-CMV per donor exposure of 0% (95% confidence interval, 0.0%-0.12%). However, 17 of 23 patients seroconverted for anti-CMV IgG, but none for anti-CMV IgM. CMV IgG seroconverters received significantly more transfusions per week than nonconverters., CONCLUSION: The risk of TT-CMV is low in high-risk CMVneg/neg HSCT patients transfused with leukoreduced blood products not tested for anti-CMV. The cause of anti-CMV IgG seroconversion is most likely passive antibody transmission by blood products., Copyright (C) 2011 Blackwell Publishing Ltd. M1 - wdn, 0417360 PB - (1) From the Institut fur Immunologie und Transfusionsmedizin; Klinik fur Innere Medizin C, Haematologie und Onkologie; Friedrich Loffler Institut fur Medizinische Mikrobiologie; and Institut fur Community Medicine, Ernst-Moritz-Arndt-Universitat, Greifsw CY - Copyright (C) 2011 Blackwell Publishing Ltd. VL - 51 SN - 0041-1132 CP - 12 L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=ovftm&NEWS=N&AN=00007885-201112010-00017 ID - 4045 ER -