TY - JOUR T1 - Detection of donor-derived CMV-specific T cells in cerebrospinal fluid in a case of CMV meningoencephalitis after cord blood stem cell transplantation. JF - International journal of hematology//Int J Hematol Y1 - 2013 A1 - Ikegame, Kazuhiro A1 - Kato, Ruri A1 - Fujioka, Tatsuya A1 - Okada, Masaya A1 - Kaida, Katsuji A1 - Ishii, Shinichi A1 - Yoshihara, Satoshi A1 - Inoue, Takayuki A1 - Taniguchi, Kyoko A1 - Tamaki, Hiroya A1 - Soma, Toshihiro A1 - Ogawa, Hiroyasu KW - *Cord Blood Stem Cell Transplantation/ae [Adverse Effects] KW - *Cytomegalovirus Infections/im [Immunology] KW - *Cytomegalovirus Infections/tm [Transmission] KW - *Cytomegalovirus/im [Immunology] KW - *Meningoencephalitis/et [Etiology] KW - *Meningoencephalitis/tm [Transmission] KW - *T-Lymphocyte Subsets/im [Immunology] KW - Cerebrospinal Fluid/cy [Cytology] KW - Cerebrospinal Fluid/im [Immunology] KW - Cytomegalovirus Infections/di [Diagnosis] KW - Cytomegalovirus/ge [Genetics] KW - Humans KW - Male KW - Meningoencephalitis/di [Diagnosis] KW - Middle Aged KW - T-Lymphocyte Subsets/me [Metabolism] KW - Tissue Donors AB - Cytomegalovirus (CMV) meningoencephalitis is a rather rare complication after allogeneic stem cell transplantation. We describe here the case of a 59-year-old man with acute myeloid leukemia who developed CMV meningoencephalitis after cord blood transplantation. The patient presented with a sudden onset of neurological symptoms, such as convulsion, on day 37. The analysis of cerebrospinal fluid (CSF) sample revealed an increase in the number of cells, which were of donor (cord blood) origin, consisting mainly of T cells. No bacteria were detected in the CSF sample. Real-time PCR analysis revealed that the CSF sample was positive for CMV, but was negative for HHV-6, adenovirus, or BK virus. The patient was diagnosed with CMV meningoencephalitis and received cidofovir. His neurological symptoms were gradually improved and completely disappeared by day 60. CMV-specific dextramer-positive CD8(+) T cells were detected in the peripheral blood and CSF samples, with the frequency being much higher in the CSF. To our knowledge, this is the first report on the appearance of CMV-specific T cells in CSF samples from a patient with CMV meningoencephalitis. Cord blood-derived CMV-specific T cells may develop early after transplantation, enter the intrathecal compartment, and likely contribute to the regulation of CMV-meningoencephalitis. M1 - a7f, 9111627 PB - Ikegame,Kazuhiro. Division of Hematology, Department of Internal Medicine, Hyogo Medical College, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan. CY - Japan VL - 97 SN - 1865-3774 CP - 2 L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=reference&D=medl&NEWS=N&AN=23271411 ID - 4043 ER -