TY - JOUR T1 - Cytomegalovirus infection in a cyclosporine-treated burn patient: case report. Y1 - 1992 A1 - Bale,J. F.,Jr A1 - Kealey,G. P. A1 - Ebelhack,C. L. A1 - Platz,C. E. A1 - Goeken,J. A. KW - AIM, IM KW - Burns / co [Complications] KW - Burns / su [Surgery] KW - Cyclosporine / ad [Administration & Dosage] KW - Cytomegalovirus / ge [Genetics] KW - Cytomegalovirus / ip [Isolation & Purification] KW - Cytomegalovirus Infections / et [Etiology] KW - Cytomegalovirus Infections / mi [Microbiology] KW - DNA, Viral / an [Analysis] KW - Humans KW - Male KW - Middle Aged KW - Skin / mi [Microbiology] KW - Skin Transplantation / ae [Adverse Effects] AB - We report the case of a 45-year-old burned man (55% total body surface area full-thickness burn) who developed symptomatic cytomegalovirus infection during cyclosporine (CSA) therapy (3 mg/kg orally) for skin transplantation. During the sixth hospital week the patient developed signs compatible with CMV infection, and CMV was recovered from the urine and sputum. Examination of skin biopsy specimens from the transplanted cadaver allograft revealed inclusion bodies compatible with CMV infection, and CMV antigens were detected by immunohistochemical testing. The CMV infection of the skin was confirmed by recovery of infectious virus and by detection of CMV nucleic acids using in situ hybridization with a biotinylated HCMV DNA probe. Restriction enzyme analysis of a urine CMV isolate and two isolates from skin demonstrated differences in DNA patterns, suggesting that the patient was infected simultaneously with two different CMV strains. CY - UNITED STATES VL - 32 CP - 2 N1 - Status: MEDLINE; Publishing Model: Journal available in: Print Citation processed from: Print; NLM Journal Code: kaf, 0376373 L2 - Case Reports. Journal Article ID - 1781 ER -