TY - JOUR T1 - The incidence and importance of bacterial contaminants of cadaveric renal perfusion fluid JF - Transplant international : official journal of the European Society for Organ Transplantation Y1 - 2005 A1 - Wakelin,S. J. A1 - Casey,J. A1 - Robertson,A. A1 - Friend,P. A1 - Jaques,B. C. A1 - Yorke,H. A1 - Rigden,S. P. A1 - Emmanuel,X. F. A1 - Pareja-Cebrian,L. A1 - Forsythe,J. L. A1 - Morris,P. J. KW - *Drug Contamination KW - *Kidney KW - *Kidney Transplantation KW - *Organ Preservation Solutions KW - *Perfusion KW - Antibiotic Prophylaxis KW - Bacteria / *isolation & purification KW - Blood / microbiology KW - Cadaver KW - Humans KW - Peritoneum / microbiology KW - Preoperative Care KW - Tissue and Organ Procurement KW - Tissue Donors AB - Infections represent a significant risk in the postoperative transplant recipient. The perfusion fluid used to perfuse and preserve the kidneys prior to transplantation represents a potential medium in which organisms can grow. The aim of this study was to determine the incidence and clinical relevance of bacterial contamination of perfusion fluid. A total of 4 centres participated in the study and 269 perfusion fluid samples were taken for microbiological analysis. Organisms were isolated from 38 out of 218 (17.4%) perfusion fluid samples taken prior to allograft implantation and 23 out of 51 (45%) samples taken at procurement. Low virulence organisms predominated although Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli were also isolated. Although infective complications were not seen in the allograft recipients, given the frequency with which contamination occurs and the variation in unit antibiotic protocols, we recommend the routine culturing of perfusion fluid to ensure that any potentially significant organisms are identified and treated appropriately. VL - 17 CP - 11 N1 - Wakelin, Sonia J Casey, John Robertson, Amanda Friend, Peter Jaques, Bryon C Yorke, Hester Rigden, Sue P Emmanuel, Xavier F S Pareja-Cebrian, Lucia Forsythe, John L R Morris, Peter J Germany Transpl Int. 2005 Jan;17(11):680-6. Epub 2004 Nov 24. ID - 1598 ER -