TY - JOUR T1 - Epstein-Barr virus-associated post-transplant lympho-proliferative disease of donor origin in liver transplant recipients JF - J Hepatol Y1 - 1997 A1 - Strazzabosco,M. A1 - Corneo,B. A1 - Iemmolo,R. M. A1 - Menin,C. A1 - Gerunda,G. A1 - Bonaldi,L. A1 - Merenda,R. A1 - Neri,D. A1 - Poletti,A. A1 - Montagna,M. A1 - Del Mistro,A. A1 - Faccioli,A. M. A1 - D'Andrea,E. KW - *Herpesvirus 4, Human KW - *Tissue Donors KW - B-Lymphocytes / pathology KW - Cell Line KW - Herpesviridae Infections / *etiology / pathology / radiography KW - Humans KW - Liver Transplantation / *adverse effects KW - Lymphoproliferative Disorders / *etiology / genetics / *virology KW - Male KW - Microsatellite Repeats KW - Middle Aged KW - Tomography, X-Ray Computed KW - Tumor Virus Infections / *etiology / pathology / radiography AB - BACKGROUND/AIMS: Post-transplant lymphoproliferative disease, a potential complication of solid organ transplantation, occurs in about 3% of orthotopic liver transplant recipients. We report the genetic and virological characterization of two cases of post-transplant lymphoproliferative disease that occurred early (4 and 6 months) after orthotopic liver transplant as large-cell non-Hodgkin's lymphomas located at the hepatic hilum. METHODS: Lymphomatous tissues were analyzed for clonality and presence of Epstein-Barr virus (EBV) sequences by Southern blot, polymerase chain reaction, and in situ hybridization techniques. RESULTS: The tumors in both cases were sustained by a clonal proliferation of B lymphocytes containing type A EBV DNA. Moreover, in situ hybridization with a digoxigenin-labeled EBV-specific probe evidenced a strong nuclear signal in most of the neoplastic cells. DNA microsatellite analysis at three different loci detected alleles of donor origin in both tumor samples, suggesting that the neoplastic B cells were of donor origin. CONCLUSIONS: EBV-infected donor B lymphocytes might be responsible for intragraft post-transplant lymphoproliferative disease in orthotopic liver transplant recipients. As 20 to 30% of post-transplant lymphomas involve the graft itself, donor-derived post-transplant lymphoproliferative disease might be more frequent than presently appreciated. Prospective studies are needed to assess its real incidence and identify possible risk factors. VL - 26 CP - 4 N1 - Strazzabosco, M Corneo, B Iemmolo, R M Menin, C Gerunda, G Bonaldi, L Merenda, R Neri, D Poletti, A Montagna, M Del Mistro, A Faccioli, A M D'Andrea, E Case Reports Research Support, Non-U.S. Gov't Denmark Journal of hepatology J Hepatol. 1997 Apr;26(4):926-34. ID - 1474 ER -