TY - JOUR T1 - Safety of sperm washing and ART outcome in 741 HIV-1-serodiscordant couples JF - Hum Reprod Y1 - 2007 A1 - Savasi,V. A1 - Ferrazzi,E. A1 - Lanzani,C. A1 - Oneta,M. A1 - Parrilla,B. A1 - Persico,T. KW - *HIV Seropositivity KW - *Reproductive Techniques, Assisted KW - Acquired Immunodeficiency Syndrome / *prevention & control KW - Female KW - Fertilization in Vitro KW - Humans KW - Insemination, Artificial, Homologous KW - Male KW - Pregnancy KW - Pregnancy Rate KW - Specimen Handling / *methods KW - Sperm Injections, Intracytoplasmic KW - Spermatozoa / *virology KW - Tissue Donors KW - Treatment Outcome KW - Viral Load AB - BACKGROUND: To evaluate the safety of sperm washing and assisted reproduction technique (ART) outcome offered to serodiscordant couples with a human immunodeficiency virus-1 (HIV-1)-positive male. METHODS: Sperm washing was performed and checked by RT-PCR on each semen sample before its fresh usage. Intrauterine insemination (IUI) or IFV/ICSI was offered according to fertility profile of each couple. Non-infected women underwent HIV testing 2 weeks before each procedure and for up to 6 months after. RESULTS: Seven hundred and forty-one couples entered the study of a possible 2011 serodiscordant couples counselled over 4 years. Superovulation and IUI were performed in 581 couples, where the pregnancy rate per cycle and pregnancy rate per couple were 19 and 78%, respectively, with multiple pregnancy rate being 4%. One hundred and sixty couples were treated by IVF/ICSI, where pregnancy rate per cycle and per couple were 22 and 41%, respectively, with multiple pregnancy rate being 10%. All female partners were still HIV-1 negative at follow-up. CONCLUSION: Sperm washing within a programme of reproductive counselling was proved to be safe in this large series of serodiscordant couples. The overall pregnancy rate (70.3%), independent of the procedure used (IUI or IVF/ICSI), justifies the effort of the medical team in setting up and implementing dedicated centres and of the individual patient in seeking a safe pregnancy. VL - 22 CP - 3 N1 - Savasi, V Ferrazzi, E Lanzani, C Oneta, M Parrilla, B Persico, T England Human reproduction (Oxford, England) Hum Reprod. 2007 Mar;22(3):772-7. Epub 2006 Nov 15. ID - 1359 ER -