TY - JOUR T1 - Dengue virus transmission from live donor liver graft Y1 - 2019 A1 - Shaji Mathew, J A1 - Menon, VP A1 - Menon, VP A1 - Mallick, S A1 - Sivasankara Pillai Thankamony Amma, B A1 - Balakrishnan, D A1 - Gopalakrishnan, U A1 - Narayana Menon, R A1 - Athira, PP A1 - Jagan, OA A1 - Surendran, S AB - Arboviral transmission through transplanted organs is rare. We report a highly probable case of dengue viral transmission during live donor liver transplantation. Fever with severe thrombocytopenia was observed in the donor and recipient within 6 and 9 days after transplantation, respectively. Dengue diagnosis was confirmed by testing blood and explant tissue from the donor and recipient using dengue-specific NAT (nucleic acid testing) and serology. Serology indicated the donor had secondary dengue infection that ran a mild course. However, the dengue illness in the recipient was severe and deteriorated rapidly, eventually proving fatal. The recipient's explant liver tissue tested negative for viral RNA indicative of a pretransplant naïve status. The prM-Envelope gene sequence analysis of the donor and recipient viral RNA identified a similar serotype (DENV1) with almost 100% sequence identity in the envelope region. Molecular phylogenetic analysis of donor and recipient viral envelope sequences with regional and local dengue strains further confirmed their molecular similarity, suggesting a probable donor-to-recipient transmission via organ transplantation. Screening of living donors for dengue virus may be considered in endemic regions. ID - 4808 ER -