02692nas a2200253   4500008004100000020001400041024001700055245011800072210006900190260025600259520165300515100002002168700002202188700001802210700002102228700001702249700001902266700001702285700002302302700001802325700003202343700001802375856004502393       2015                        eng d  a0041-1132  awdn, 041736000aTransmission of human immunodeficiency virus Type-1 by fresh-frozen plasma treated with methylene blue and light.0 aTransmission of human immunodeficiency virus Type1 by freshfroze  a(C) 2015 John Wiley & Sons, Ltdb(1)Valencian Regional Blood Transfusion Center, Valencia, Spain(2)Joint Research Unit "Infection and Health" FISABIO-Universitat de Valencia and CIBER in Epidemiology and Public Health (CIBERESP), Valencia, Spainc20153 aBACKGROUND: The risk of transfusion-transmitted infection (TTI) has been minimized by introduction of nucleic acid testing (NAT) and pathogen inactivation (PI). This case report describes transmission of human immunodeficiency virus Type 1 (HIV-1) to two recipients despite these measures., STUDY DESIGN AND METHODS: In March 2009 a possible TTI of HIV-1 was identified in a patient that had received pooled buffy coat platelet concentrate (BC-PLT) in November 2005. The subsequent lookback study found two more patients who had received methylene blue (MB)-treated fresh-frozen plasma (FFP) and red blood cells (RBCs) from the same donation. In November 2005 the donor had tested negative for both HIV antibodies and HIV-1 RNA by 44 minipool (44 MP) NAT. Repository samples of this donation and samples from the recipients were used for viral load (VL) and sequence analysis., RESULTS: HIV-1 RNA was detectable by individual donation (ID)-NAT in the repository sample from the 2005 window period donation and a VL of 135 copies/mL was measured. HIV-1 infection was confirmed in both recipients of both BC-PLT (65 mL of plasma) and MB-FFP (261 mL of plasma), but not in the patient that had received 4-week-old RBCs (20 mL of plasma). The sequence analysis revealed a close phylogenetic relationship between the virus strains isolated from the donor and recipients, compatible with TTI., CONCLUSIONS: Approximately 17,600 and 4400 virions in the MB-FFP and BC-PLT were infectious, but 1350 virions in the RBCs were not. ID-NAT would have prevented this transmission, but the combination of MP-NAT and MB-PI did not., (C) 2015 John Wiley & Sons, Ltd1 aAlvarez, Manuel1 aLuis-Hidalgo, Mar1 aBracho, Maria1 aBlanquer, Amando1 aLarrea, Luis1 aVillalba, Jose1 aPuig, Nieves1 aPlanelles, Dolores1 aMontoro, Jose1 aGonzalez-Candelas, Fernando1 aRoig, Roberto  uhttps://notifylibrary.org/reference/4456