02556nas a2200205   4500008004100000020001400041245012600055210006900181260022500250300001600475490000800491520169300499100001802192700001702210700001602227700001902243700002002262700002302282856004502305       2009                        eng d  a0022-189900aAn Assessment of Donor-to-Recipient Transmission Patterns of Human Cytomegalovirus by Analysis of Viral Genomic Variants.0 aAssessment of DonortoRecipient Transmission Patterns of Human Cy  a(C) Copyright Oxford University Press 2009.b(1)Provincial Laboratory for Public Health (Microbiology) and(2)Division of Infectious Diseases, Department of Medicine, University of Alberta, Edmonton, Alberta, Canadac2009  a1621 - 16280 v1993 aBackground: We studied human cytomegalovirus (CMV) donor-to-recipient transmission patterns in organ transplantation by analyzing genomic variants on the basis of CMV glycoprotein B (gB) genotyping., Methods: Organ transplant recipients were included in the study if they had CMV viremia, if they had received an organ from a CMV-seropositive donor, and if there was at least 1 other recipient of an organ from the same donor who developed CMV viremia. Genotypes (gB1-4) were determined by real-time polymerase chain reaction., Results: Forty-seven recipients of organs from 21 donors developed CMV viremia. Twenty-three recipients had a pretransplant donor/recipient (D/R) CMV serostatus of D+/R+, and 24 had a serostatus of D+/R-. The prevalences of genotypes in recipients were as follows: for gB1, 51% (n=24); for gB2, 19% (n=9); for gB3, 9% (n=4); for gB4, 0% (n=0); and for mixed infection, 21% (n=10). Recipients of an organ from a common donor had infection with CMV of the same gB genotype in 12 (57%) of 21 instances. Concordance between genotypes was higher among seronegative (i.e., D+/R-) recipients than among seropositive (D+/R+) recipients, although discordances resulting from the transmission of multiple strains were seen. In seropositive recipients, transmission of multiple strains from the donor could not be differentiated from reactivation of a recipient's own strains., Conclusion: Our analysis of strain concordance among recipients of organs from common donors showed that transmission of CMV has complex dynamic patterns. In seropositive recipients, transmission or reactivation of multiple CMV strains is possible., (C) Copyright Oxford University Press 2009.1 aManuel, Oriol1 aPang, Xiaoli1 aHumar, Atul1 aKumar, Deepali1 aDoucette, Karen1 aPreiksaitis, Jutta  uhttps://notifylibrary.org/reference/4155