02624nas a2200229   4500008003900000245013300039210006900172260000800241300001400249490000700263520193500270100001602205700001302221700001402234700001802248700001802266700001902284700001502303700001502318700001602333856004502349       1998                          d00aAcceptance and feasibility of peripheral stem cell mobilisation compared to bone marrow collection from healthy unrelated donors0 aAcceptance and feasibility of peripheral stem cell mobilisation   cJun  aS25 - S280 v213 aAllogeneic peripheral blood stem cell transplantation leads to an earlier engraftment compared to BMT, The feasibility, acceptance and long-term side-effects of G-CSF mobilisation of PBSC in unrelated healthy donors needs to be evaluated, Forty unrelated healthy donors received G-CSF in a dose of 10 mu g/kg bodyweight for 5 days and two aphereses were performed. The donors were monitored prospectively, The data were compared to bone marrow harvests from unrelated donors. Almost all stem cell donors reported some side-effects due to Filgrastim application. Bone pain (32), headache (20), chest pain (two) and night sweats (one) were complained of. By taking analgesics, the pain was relieved in most cases. No donor discontinued the filgrastim application. Bone pain and headache resolved within 2-4 days after termination of Filgrastim application. There was, as expected, a seven-fold increase in the number of total WBCs. There were no significant changes of platelet counts during G-CSF application. After 4 weeks haemoglobin concentration and platelet counts showed no significant differences compared to baseline values. The aphereses were mostly tolerated very well. Eighteen donors reported paraesthesia, one donor developed dizziness, two complained of nausea and vomiting. There was a significant decrease in platelet count (242 before, 98 x 10(9)/l after aphereses), Autologous platelets were transfused after the second aphereses in four donors. These data were compared to data from 245 unrelated bone marrow donors, who had on average, 14 days bone pain and tiredness after donation. The G-CSF mobilisation and apheresis of peripheral blood stem cells is an alternative to traditional bone marrow harvesting in unrelated healthy donors. It is well tolerated and the duration of side-effects on average is shorter than after the surgical procedure. So far no long-term effects have been observed in the followup.1 aOrdemann, R1 aHolig, K1 aWagner, K1 aRautenberg, U1 aBornhauser, M1 aKroschinsky, F1 aSchafer, J1 aSchuler, U1 aEhninger, G  uhttps://notifylibrary.org/reference/1106