02495nas a2200277   4500008003900000245011800039210006900157260000800226300000600234490000700240520171900247100001601966700001501982700001401997700002002011700001602031700001602047700001402063700001502077700001802092700001402110700001402124700001802138700001602156856004502172       2017                          d00aDonor-Derived Mycoplasma hominis and an Apparent Cluster of M. hominis Cases in Solid Organ Transplant Recipients0 aDonorDerived Mycoplasma hominis and an Apparent Cluster of M hom  cOct  a40 v653 aBackground:  Invasive and disseminated Mycoplasma hominis infections are well recognized but uncommon complications in solid organ transplant recipients. In a single center, a cluster of M. hominis infections were identified in lung transplant recipients from the same thoracic intensive care unit (ICU). We sought to determine the source(s) of these infections. Methods:  Medical records of the donor and infected transplant recipients were reviewed for clinical characteristics. Clinical specimens underwent routine processing with subculture on Mycoplasma-specific Hayflick agar. Mycoplasma hominis identification was confirmed using sequencing of the 16S ribosomal RNA gene. Mycoplasma hominis isolates were subjected to whole-genome sequencing on the Illumina NextSeq platform. Results:  Three lung transplant recipients presented with invasive M. hominis infections at multiple sites characterized by purulent infections without organisms detected by Gram staining. Each patient had a separate donor; however, pretransplant bronchoalveolar lavage fluid was only available from the donor for patient 1, which subsequently grew M. hominis. Phylo- and pangenomic analyses indicated that the isolates from the donor and the corresponding recipient (patient 1) were closely related and formed a distinct single clade. In contrast, isolates from patients 2 and 3 were unrelated and divergent from one another. Conclusions:  Mycoplasma hominis should be considered a cause of donor-derived infection. Genomic data suggest donor-to-recipient transmission of M. hominis. Additional patients co-located in the ICU were found to have genetically unrelated M. hominis isolates, excluding patient-to-patient transmission.1 aSmibert, OC1 aWilson, HL1 aSohail, A1 aNarayanasamy, S1 aSchultz, MB1 aBallard, SA1 aKwong, JC1 ade Boer, J1 aMorrissey, CO1 aPeleg, AY1 aSnell, GI1 aParaskeva, MA1 aJenney, AWJ  uhttps://notifylibrary.org/reference/4787