01971nas a2200157   4500008003900000245008300039210006900122260000800191300000700199490000700206520151900213100001201732700001201744700001201756856004501768       2009                          d00aClinical Considerations on Contamination Rates of Cryopreserved Autologous Fat0 aClinical Considerations on Contamination Rates of Cryopreserved   cNov  a850 v363 aPURPOSE: Autologous fat graft is a widely accepted technique used for soft tissue augmentation. Nonetheless, the use of fat graft is limited due to unpredictable survival rates and repeated grafting. To avoid repeated grafting, cryopreserved fat graft technique has recently been widely used. On the other hand, the number of patients with chronic infection (who received cryopreserved fat injection) has currently been increasing. This study is focused on the safety of cryopreserved fat injection from the infection. METHODS: We collected 150 samples from local aesthetic clinics to examine the safety of cryopreserved autologous fat. To test for microbacterial contaminations of the cryopreserved fat specimens, microbacterial cultures & antibiotics sensitivity tests were performed. Then, we examined possible correlation between the preservation period and donor sites, focused on the results of microbacterial culture. RESULTS: Cultures were positive for Staphylococcus epidermidis in 5 samples (methicillinresistant Staphylococcus epidermidis in 4 samples), Micrococcus species in 3 samples. An average duration of preservation was 191 days and there found no significant correlation between the duration of preservation and microbacterial growth. CONCLUSION: Staphylococcus epidermidis was the leading cause of cryopreserved fat contamination, and the resistance to methicillin is common. Based on the above results, aseptic handling of fat during harvesting and preservation appeared to be most important.1 aKim, JT1 aSeo, WJ1 aKim, YH  uhttps://notifylibrary.org/reference/4730