@article {1415,
	title = {Transmission of human immunodeficiency virus type 1 from a seronegative organ and tissue donor},
	journal = {N Engl J Med},
	volume = {326},
	year = {1992},
	note = {Simonds, R J Holmberg, S D Hurwitz, R L Coleman, T R Bottenfield, S Conley, L J Kohlenberg, S H Castro, K G Dahan, B A Schable, C A Case Reports United states The New England journal of medicine N Engl J Med. 1992 Mar 12;326(11):726-32.},
	month = {40969},
	pages = {726 - 32},
	edition = {33675},
	abstract = {BACKGROUND: Since 1985, donors of organs or tissues for transplantation in the United States have been screened for human immunodeficiency virus type 1 (HIV-1), and more than 60,000 organs and 1 million tissues have been transplanted. We describe a case of transmission of HIV-1 by transplantation of organs and tissues procured between the time the donor became infected and the appearance of antibodies. The donor was a 22-year-old man who died 32 hours after a gunshot wound; he had no known risk factors for HIV-1 infection and was seronegative. METHODS: We reviewed the processing and distribution of all the transplanted organs and tissues, reviewed the medical histories of the donor and HIV-1-infected recipients, tested stored donor lymphocytes for HIV-1 by viral culture and the polymerase chain reaction, and tested stored serum samples from four organ recipients for HIV-1 antigen and antibody. RESULTS: HIV-1 was detected in cultured lymphocytes from the donor. Of 58 tissues and organs obtained from the donor, 52 could be accounted for by the hospitals that received them. Of the 48 identified recipients, 41 were tested for HIV-1 antibody. All four recipients of organs and all three recipients of unprocessed fresh-frozen bone were infected with HIV-1. However, 34 recipients of other tissues--2 receiving corneas, 3 receiving lyophilized soft tissue, 25 receiving ethanol-treated bone, 3 receiving dura mater treated with gamma radiation, and 1 receiving marrow-evacuated, fresh-frozen bone--tested negative for HIV-1 antibody. Despite immunosuppressive chemotherapy, HIV-1 antibody appeared between 26 and 54 days after transplantation in the three organ recipients who survived more than four weeks. CONCLUSIONS: Although rare, transmission of HIV-1 by seronegative organ and tissue donors can occur. Improvements in the methods used to screen donors for HIV-1, advances in techniques of virus inactivation, prompt reporting of HIV infection in recipients, and accurate accounting of distributed allografts would help to reduce further this already exceedingly low risk.},
	keywords = {*HIV Seropositivity, *HIV-1 / isolation \& purification, *Tissue Donors, Acquired Immunodeficiency Syndrome / *transmission, Adult, Bone Transplantation / adverse effects, Cells, Cultured, Corneal Transplantation / adverse effects, Heart Transplantation / adverse effects, HIV Antibodies / analysis, Humans, Kidney Transplantation / adverse effects, Liver Transplantation / adverse effects, Lymphocytes / microbiology, Male, Organ Transplantation / *adverse effects, Tissue Banks / *standards, United States},
	issn = {0028-4793 (Print) 0028-4793 (Linking)},
	doi = {10.1056/NEJM199203123261102},
	url = {internal-pdf://Simonds - Transmission of HIV-3798305281/Simonds - Transmission of HIV.pdf},
	author = {Simonds,R. J. and Holmberg,S. D. and Hurwitz,R. L. and Coleman,T. R. and Bottenfield,S. and Conley,L. J. and Kohlenberg,S. H. and Castro,K. G. and Dahan,B. A. and Schable,C. A.}
}
