@article {829,
	title = {Multi-organ donor transmission of hepatitis C virus to five solid organ transplant recipients and lack of transmission to corneal transplant recipients},
	journal = {Clin Diagn Virol},
	volume = {3},
	year = {1995},
	note = {Krajden, M Bishai, F Quan, C Mahony, J Brunton, J Rootman, D Zhao, J Lau, W Snell, G Maurer, J Kesten, S Colby, D Netherlands Clinical and diagnostic virology Clin Diagn Virol. 1995 Feb;3(2):113-21.},
	month = {Feb},
	pages = {113 - 21},
	edition = {34731},
	abstract = {BACKGROUND: A multi-organ donor seronegative for hepatitis C virus (HCV) by 1st generation enzyme immunoassay (EIA) supplied 5 solid organs and 2 corneas to 7 recipients. This donor was retrospectively shown to be 2nd generation HCV EIA-positive and polymerase chain reaction (PCR)-positive. All 5 solid organ recipients but none of the corneal recipients developed HCV infection. OBJECTIVES: To demonstrate the discordance between serological and PCR-based HCV detection in solid organ transplant recipients and the lack of transmission of HCV to the two corneal transplant recipients. STUDY DESIGN: All 5 solid organ recipients were retrospectively shown to be HCV PCR-negative and -seronegative pre-transplant and HCV PCR-positive post-transplant. Serial serum samples on 3 recipients were evaluated by 2nd generation EIA, a prototypic structural and non-structural dual bead assay (EIA-SA, Abbott), the Chiron Recombinant Immunoblot Assay, 2nd generation (RIBA-2), and the Chiron RIBA HCV Test System Strip Immunoblot Assay 3.0 (RIBA-3, Chiron). RESULTS: The dual bead EIA-SA and RIBA-3 were able to detect HCV seroconversion approximately 6 months earlier than the 2nd generation EIA in 2 recipients, and in 1 recipient only PCR detected infection within the first 10 months. There was no evidence of HCV transmission to the corneal recipients. CONCLUSIONS: Although third generation assays such as the RIBA-3 and EIA-SA narrowed the window of HCV seronegativity in transplant recipients compared with the 2nd generation EIA, PCR was the most sensitive method of detecting acute HCV infection. Despite transmission of HCV to all of the solid organ recipients HCV was not transmitted to the corneal transplant recipients.},
	issn = {0928-0197 (Print) 0928-0197 (Linking)},
	doi = {092801979400031O [pii]},
	url = {internal-pdf://Krajden - Hepatitis C-2086354945/Krajden - Hepatitis C.pdf},
	author = {Krajden,M. and Bishai,F. and Quan,C. and Mahony,J. and Brunton,J. and Rootman,D. and Zhao,J. and Lau,W. and Snell,G. and Maurer,J. and Kesten,S. and Colby,D.}
}
