@article {4770,
	title = {Multicenter Prospective Study for Laboratory Diagnosis of HHV8 Infection in Solid Organ Donors and Transplant Recipients and Evaluation of the Clinical Impact After Transplantation},
	volume = {101},
	year = {2017},
	month = {Aug},
	pages = {1944},
	chapter = {1935},
	abstract = {<p>BACKGROUND: We performed serological and molecular pretransplant screening in solid organ transplant (SOT) donors and recipients in north central Italy and a surveillance program for human herpesvirus 8 (HHV8) infection after transplant, aiming to establish an optimal management of HHV8 infection in SOT recipients. METHODS: For pretransplant HHV8 screening in both donors and recipients, 6 serological (4 indirect immunofluorescent assays [IFA] and 2 enzyme-linked immunosorbent assays-both HHV8 lytic and latent antigen based) and 2 molecular assays were used. A reference standard to identify HHV8-positive patients was defined by at least 2 positive assays. All transplant patients at risk to develop HHV8-related disease underwent virological posttransplant monitoring by quantitative real-time polymerase chain reaction (PCR) assay. RESULTS: Human herpesvirus 8 seroprevalence was 4\% (10/249) in donors and 18\% (93/517) in organ recipients. The best performance was obtained by 2 lytic antigen-based IFAs that showed almost perfect agreement to the reference standard (0.943 and 0.931 Cohen kappa). Human herpesvirus 8-DNA was detected in 6.8\% and 2.9\% of HHV8-seropositive donor samples by in-house nested PCR and quantitative real-time PCR assays, respectively. After transplant, 3 (25\%) of 12 HHV8-mismatch patients (seropositive donor/seronegative recipient) developed a primary infection, one of whom developed a lethal nonmalignant illness. Two of 93 HHV8-seropositive recipients (2.1\%) had viral replication in posttransplant period, one of whom developed Kaposi sarcoma. CONCLUSIONS: Serological assays, specifically lytic IFAs, were the best methodological approach to identify HHV8-infected SOT donors and recipients. A very low incidence (1.9\%) of posttransplant HHV8-related disease was observed.</p>},
	doi = {10.1097/TP.0000000000001740},
	author = {Chiereghin, A and Barozzi, P and Petrisli, E and Piccirilli, G and Gabrielli, L and Riva, G and Potenza, L, Cappelli, G and De Ruvo, N, Libri, I and Maggiore, U, Morelli, MC and Potena, L, Todeschini, P, Gibertoni, D, Labanti, M, Sangiorgi, G, La Manna, G, Pinna, AD and Luppi, M and Lazzarotto, T}
}
