| Title | Effect of contaminated preprocessed semen on fertilization rate and embryo quality in assisted reproductive techniques. |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Krissi H, Orvieto R, Ashkenazi J, Gilboa Y, Shalev J, Moscovitch I, Bar-Hava I |
| Journal | Gynecol Endocrinol |
| Volume | 18 |
| Issue | 2 |
| Pagination | 63 - 7 |
| Date Published | Feb |
| ISSN | 0951-3590 |
| Accession Number | 15195496 |
| Keywords | Adult, Embryo, Mammalian, Female, Fertilization, Fertilization in Vitro, Gram-Positive Bacteria, Handwashing, Humans, Male, Pregnancy, Prospective Studies, Semen, Sperm Injections, Intracytoplasmic |
| Abstract | We aimed to identify the sources and prevalence of semen contamination from mastrubation and determine the effect of bacterospermia on fertilization rate and embryo quality in standard in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). This was a prospective controlled study, in an IVF unit of a university teaching hospital, of 93 consecutive couples undergoing IVF-embryo transfer cycles. We evaluated handwashing; semen collection and processing; and assisted reproductive technology using semen provided by masturbation. The main outcome measures were presence and type of micro-organisms in the semen samples and embryo culture medium; the effect of hand washing on rate of contamination; and the effect of semen contamination on fertilization rate and embryo quality. The first consecutive 52 men of the 93 couples were not instructed to wash their hands before masturbation, and the remainder were so instructed. Forty-nine semen cultures (94.2%) in the first group were contaminated compared to only 16 (39%) in the second (p |
| Notify Library Reference ID | 837 |
